The investigation was undertaken in the Department of Horticulture, College of Agriculture, Dapoli during 2009. The investigation involved completely randomized design with 4 treatments viz, T1- Cel -fresh 0.18% Tablet (1-MCP)-1Tablet/1M3, T2- Cel -fresh 0.18% Tablet (1-MCP)-1Tablet / 2M3 , T3- Cel -fresh 0.18% Tablet (1-MCP)-1Tablet / 3M.3 airtight fumigation chamber (fruits were kept for 6 hours), T4-Control. 1-methylcyclopropene resulted in delaying ripening process by 6 to 7 days which was least in T4 (control) at cold storage. Under cold storage T2 recorded highest T. S. S. (18.700 Brix), reducing Sugar (3.95 %), total sugar (13.48 %), ascorbic acid (44.43 mg/100g) as compared to control and the rest of the treatments. The highest pulp acidity (0.38 %) was recorded in T3 as compared to rest of the treatments at ripe stage. From the present investigation it can be concluded that 1-methylcyclopropene was found to be a potent inhibitor of ripening in mango Cv. Alphonso which can an increasing shelf life of fruits, retarding biochemical change and enhancing shelf life of fruits without loss in quality at cold storage temperature. The chemical analysis and sensory qualities of Alphonso mango fruits cold storage conditions indicated that Cel-fresh 0.18% Tablet (1-MCP)-1Tablet/2M3 treatment (T2) was superior over control and better than other treatments.