This study was conducted to know the effect of Auxin and Cytokinin type and its concentration for invitro
propagation of Prunus domestica L.var. Pioneer explants. Pioneer explants were sterilized by using different
concentration of sodium hypochlorite and mercuric chloride, and then cultured on Murashige and Skoog media
supplemented with different concentration of BAP or KIN alone and in combination with IBA. The shoots were
transferred to half strength MS medium supplemented with varying concentrations of IBA or IAA for root growth and
development. Explants disinfected with sodium hypochlorite (2%) for 15 minutes and mercuric chloride (0.1 %) for 7
minutes has highest significant survival value. The highest growth and development rate of shoots was obtained on
media with BAP and poor multiplication was achieved on media with KIN. From different combinations of KIN or BAP
with IBA, the highest shoot induction was observed on MS media supplemented with 0.5 mg/L BAP in combination with
0.1 mg/L IBA with an average number of 3.18 ± 0.58 shoots per explants and 3.36 ± 0.29 cm average shoot length.
The highest rooting was observed on 1.0 mg/L IBA with an average number of 4.05 ± 1.2 roots per shoot and 3.66 ± 0.1
cm average root length.
