Includes bibliographical references and index

Cover -- Contents -- Contributors -- Preface -- Preface to the Third Edition -- Preface to the Second Edition -- Preface to the First Edition -- Part I: Instrumentation/Fluorochromes -- Chapter 1. Optimization of Emission Optics for Multicolor Flow Cytometry -- I. Introduction -- II. Fluorochromes -- III. Hardware Components Affecting Light Collection -- IV. Maximizing Signal Intensities -- V. Maximizing Signal Collection Quality -- VI. Emission Collection Optimization -- VII. Testing Emission Optics -- References -- Chapter 2. Two-Photon Tissue Cytometry -- I. Introduction -- II. Technical Development of Two-Photon Tissue Cytometry -- III. Experimental Demonstration of Two-Photon Tissue Cytometry -- IV. Future Outlook -- V. Conclusion -- References -- Chapter 3. Confocal Microscopy: Quantitative Analytical Capabilities -- I. Introduction -- II. Cartesian Coordinates, Distance, Size, Shape, and Volume in Confocal Microscopy --^

III. Measurements of Fluorescence Intensity in Confocal Microscopy -- IV. Time Resolution in Confocal Microscopy -- V. Confocal Techniques Based on Measurements of Fluorescence Intensity vs Time -- VI. Spectrally Resolved Confocal Microscopy -- VII. Conclusion -- References -- Chapter 4. Surface-Plasmon-Coupled Emission. New Technology for Studying Molecular Processes -- I. Introduction -- II. Overview of Surface Plasmon Resonance -- III. Surface-Plasmon-Coupled Emission -- IV. Experimental Studies of Surface-Plasmon-Coupled Emission -- V. Practical Applications -- VI. Conclusion -- References -- Chapter 5. Cytometry of Fluorescence Resonance Energy Transfer -- I. Introduction -- II. Theory of FRET -- III. Measuring FRET -- IV. Applications -- V. Perspectives -- VI. Conclusions -- References -- Chapter 6. The Rainbow of Fluorescent Proteins -- I. The Fluorescent Proteins -- II. Flow Analysis Using Fluorescent Proteins -- III. Flow Sorting Using Fluorescent Proteins --^

IV. Conclusion -- References -- Chapter 7. Labeling Cellular Targets with Semiconductor Quantum Dot Conjugates -- I. Introduction -- II. Selection of QDs and Their Conjugates -- III. Labeling of Fixed Cells for Fluorescence Microscopy -- IV. Measurements on Living Cells Using QD Conjugates -- V. Conclusion -- References -- Chapter 8. Next-Generation Laser Scanning Cytometry -- I. Introduction -- II. LSC-Hybrid Technology -- III. Description of the LSC Platform -- IV. Selected Applications -- V. Utility of Solid-Phase Imaging Cytometry in Life Science Research and Drug Discovery -- References -- Part II: General Techniques -- Chapter 9. Biohazard Sorting -- I. Introduction -- II. Critical Aspects of the Procedure -- III. Applications and Future Directions -- References -- Chapter 10. Guidelines for the Presentation of Flow Cytometric Data -- I. Introduction -- II. General Principles of Graphical Presentation -- III. Statistics -- IV. Subset Analysis -- V. Conclusion -- Reference

Description based on print version record

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