Proteins play a central role in all biological functions. This practical work explains how the same 20 amino acids can be used to produce such diverse properties and functional roles, the secret being in their three-dimensional structure
Includes bibliographical references and index
Basic aspects of polypeptide structure -- Covalent structures of proteins -- Non-covalent forces in protein structure -- Electrostatic interactions -- van der Waals interactions -- Hydrogen bonds -- Hydrophobic interactions -- Amino acid residues -- Primary structures of proteins -- Three-dimensional aspects of polypeptide structure -- Conformations of polypeptides -- Regular polypeptide structures -- Structures of fibrous proteins -- Silk fibroin -- Collagen -- Coiled-coils -- Three-dimensional structures of proteins -- Determining protein structure -- CD spectroscopy -- X-ray diffraction -- NMR spectroscopy -- Structural organization in globular proteins -- Tertiary structure -- Protein interiors and exteriors -- Secondary structure -- Loops and turns -- Disulphide bonds -- Domains -- Supersecondary structure and folding patterns -- Quaternary structure -- Flexibility in protein structures -- Evolutionarily related proteins -- Predicting protein structure -- Protein design -- Proteins in solution and in membranes -- Proteins in aqueous solution -- Aqueous solubility -- Ionization
(cont) Membrane proteins -- Stability of the folded state -- Unfolding -- Thermodynamics of unfolding -- Rationalizing protein stability -- Other factors that affect protein stability -- Protein folding -- General characteristics of protein folding -- Identifying folding intermediates -- Biosynthetic folding -- Ligand binding and protein function -- Ligand binding -- Studying protein-ligand interactions -- General properties of protein-ligand interactions -- Relationship between protein structure and ligand binding -- Calcium-binding proteins -- Redox proteins -- Nucleotide-binding proteins -- DNA-binding proteins -- Helix-turn-helix proteins -- Zinc-containing DNA recognition elements -- Other DNA-binding motifs -- Regulation of ligand binding -- Allosteric regulation -- Reversible covalent modification
